LncRNA HCG11 Suppresses Cell Proliferation and Promotes Apoptosis via Sponging miR-224-3p in Non-Small-Cell Lung Cancer Cells

Guige Wang; Lei Liu; Jiaqi Zhang; Cheng Huang; Yeye Chen; Wenliang Bai; Yanqing Wang; Ke Zhao; Shanqing Li

doi:10.2147/OTT.S244181

LncRNA HCG11 Suppresses Cell Proliferation and Promotes Apoptosis via Sponging miR-224-3p in Non-Small-Cell Lung Cancer Cells

Onco Targets Ther. 2020 Jul 3:13:6553-6563. doi: 10.2147/OTT.S244181. eCollection 2020.

Authors

Guige Wang^{1

2}, Lei Liu^{1

2}, Jiaqi Zhang^{1

2}, Cheng Huang^{1

2}, Yeye Chen^{1

2}, Wenliang Bai^{1

2}, Yanqing Wang^{1

2}, Ke Zhao^{1

2}, Shanqing Li^{1

2}

Affiliations

¹ Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing 100730, People's Republic of China.
² Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, People's Republic of China.

Abstract

Introduction: Studies have found that Lnc-HCG11 is an important regulator of cancer. However, the function of Lnc-HCG11 in NSCLC is not known. Therefore, this experimental design was based on Lnc-HCG11 to explore the pathogenesis of NSCLC.

Methods: RT-qPCR was used to detect the expression of Lnc-HCG11 and miR-224-3p in NSCLC. The effects of Lnc-HCG11 and miR-224-3p on proliferation and apoptosis of NSCLC cells were detected by CCK-8 assay, Edu assay and Annexin V-FITC/PI assay. Target gene prediction and screening, luciferase reporter assays were used to verify downstream target genes for lnc-HCG11 and miR-224-3p. Western blotting was used to detect the protein expression of caspase-3. The tumor changes in mice were detected by in vivo.

Results: Lnc-HCG11 was significantly reduced in NSCLC. Lnc-HCG11 significantly inhibited cell proliferation of NSCLC cells and induced apoptosis. miR-224-3p was significantly elevated in the NSCLC cell line. Moreover, miR-224-3p significantly increased cell proliferation and inhibited apoptosis of NSCLC cells. Furthermore, Lnc-HCG11 was negatively correlated with miR-224-3p expression. Lnc-HCG11 over-expression was up-regulated the expression levels of c-caspase-3 and caspase-3. Finally, the results of in vivo animal models confirmed that Lnc-HCG11 inhibited tumor growth by modulating the miR-224-3p/c-caspase-3 axis.

Conclusion: Lnc-HCG11 could inhibit the progression of NSCLC by modulating the miR-224-3p/caspase-3 axis, and Lnc-HCG11 may be a potential therapeutic target for NSCLC.

Keywords: Lnc-HCG11; apoptosis; miR-224-3p; non-small-cell lung cancer; proliferation.