Evaluation on the separated effect of 13 hemoglobin variants by a new automatic Hba1c analyzer

J Clin Lab Anal. 2020 Oct;34(10):e23446. doi: 10.1002/jcla.23446. Epub 2020 Jul 12.

Abstract

Objective: To evaluate the effect of a new type of automatic glycated hemoglobin analyzer on the separation of abnormal hemoglobin.

Methods: Samples diagnosed as hemoglobin variants by capillary electrophoresis and gene testing were selected, and HbA1c analyzer was used for separation and detection.

Results: A total of 13 hemoglobin variants in 40 samples could be separated from the normal peaks.

Conclusions: The variant mode of hemoglobin HbA1c can identify a variety of hemoglobin variants, and the type of variants can be preliminarily determined according to the retention time and characteristic peak shape of the variants.

Keywords: HbA1c; hemoglobin variant; high-performance liquid chromatography.

Publication types

  • Evaluation Study

MeSH terms

  • Automation
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Capillary / instrumentation*
  • Electrophoresis, Capillary / methods*
  • Glycated Hemoglobin / genetics*
  • Glycated Hemoglobin / isolation & purification*
  • Humans
  • Thalassemia / blood
  • Time Factors

Substances

  • Glycated Hemoglobin A