Despite, different medicinal phyto compounds giving an inexhaustible variety of anticancer drugs, potent signalling mechanism of leads the key successes of anticancer agent, anti-inflammatory, induction of apoptosis, and antiangiogenic. The current study was conducted to estimate the effect of syringic acid (SA) on tumor necrosis factor-α (TNF-α)-mediated nuclear factor-κB (NF-κB) signaling pathways, inducing apoptosis and angiogenic signaling pathways in a hamster model by preneoplastic stages, histological, immunohistochemistry and immunoblots analysis. Hamsters were given oral cancer by painting 0.5% 7,12-dimethylbenz[a]anthracene (DMBA) for 10 weeks. The DMBA-painted hamsters were treated with an effective dose (50 mg/kg body weight) of SA for 14 weeks. The results revealed that oral preadministration of SA to DMBA-treated hamster oral tumorigenesis significantly increased Bcl-2-associated X protein, caspases-3 and -9, and reduced B-cell lymphoma protein 2 and inflammatory cyclooxygenase-2 (COX-2), inducible nitric oxide synthase, and TNF-α expression through NF-κB, and angiogenic vascular endothelial growth factor markers. Taken together, the current study suggests that SA prevents the DMBA-induced hamster buccal pouch carcinogenesis by triggering intrinsic apoptotic pathway via abrogation of the downstream signaling molecules such as COX-2, NF-κB, and TNF-α. This type of preventive strategy based on animal study will offer a means to design chemoprevention trials for humans.
Keywords: DMBA; angiogenesis; apoptosis; inflammation; oral cancer; syringic acid.
© 2020 Wiley Periodicals LLC.