Electrochemical assays for determination of H2O2 and prostate-specific antigen based on a nanocomposite consisting of CeO2 nanoparticle-decorated MnO2 nanospheres

Mikrochim Acta. 2020 Jul 6;187(8):428. doi: 10.1007/s00604-020-04403-7.

Abstract

A nanocomposite consisting of CeO2 nanoparticle-decorated MnO2 nanospheres (CeO2@MnO2) was synthesized for the first time via a hydrothermal method. CeO2@MnO2 was exploited to construct an electrochemical assays for detecting H2O2 and prostate-specific antigen (PSA) with square wave voltammetry (SWV). The electrochemical results proved that CeO2@MnO2 owned a better electrocatalytic effect towards H2O2 reduction than pure MnO2 NS and CeO2 NP due to the synergistic effect between MnO2 NS and CeO2 NP. Under optimized conditions, CeO2@MnO2-based assay can be applied to detect H2O2 in the range 1 to 3.0 × 103 μmol L-1. The label-free electrochemical immunoassay based on CeO2@MnO2 displayed linearly with concentrations of PSA from 0.005 to 50.0 ng mL-1. The electrochemical assays also possessed acceptable sensitivity, selectivity, and stability. The study showed that CeO2@MnO2 hold great potential as a biosensing platform and the clinical determination of tumor markers in human serum. Graphical abstract A nanocomposite consisting of CeO2 nanoparticles decorated MnO2 nanospheres (CeO2 @MnO2) was firstly synthesized via a hydrothermal method. CeO2@MnO2 was firstly exploited to construct electrochemical assays for detecting H2O2 and prostate-specific antigen (PSA) with square wave voltammetry (SWV), respectively. The electrochemical results proved that CeO2@MnO2 owned better electrocatalysis towards H2O2 reduction than pure MnO2 NS and CeO2 NP due to the synergistic effect between MnO2 NS and CeO2 NP. Under optimized conditions, CeO2@MnO2 based assay relative to the H2O2 system can be applied to detect H2O2 with range from 1 to 3.0 × 103 μmol L-1. The label-free electrochemical immunoassay based on CeO2@MnO2 relative to the H2O2 system displayed linearly with concentrations of PSA from 0.005 to 50.0 ng mL-1. The electrochemical assays also possessed acceptable sensitivity, selectivity and stability. The study showed that CeO2@MnO2 hold great potential for biosensing platform and the clinic determination of tumor markers in human serum.

Keywords: CeO2 nanoparticles; CeO2@MnO2; Electrochemical immunoassay; H2O2; Hydrothermal method; MnO2 nanosphere; Prostate-specific antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Immobilized / immunology
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / immunology
  • Biosensing Techniques / methods
  • Catalysis
  • Cerium / chemistry
  • Electrochemical Techniques / methods
  • Humans
  • Hydrogen Peroxide / analysis*
  • Hydrogen Peroxide / chemistry
  • Immunoassay / methods
  • Limit of Detection
  • Manganese Compounds / chemistry
  • Metal Nanoparticles / chemistry*
  • Nanocomposites / chemistry*
  • Nanospheres / chemistry
  • Oxidation-Reduction
  • Oxides / chemistry
  • Prostate-Specific Antigen / blood*
  • Prostate-Specific Antigen / immunology

Substances

  • Antibodies, Immobilized
  • Biomarkers, Tumor
  • Manganese Compounds
  • Oxides
  • Cerium
  • ceric oxide
  • Hydrogen Peroxide
  • Prostate-Specific Antigen
  • manganese dioxide