A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection

Methods Mol Biol. 2020:2164:129-143. doi: 10.1007/978-1-0716-0704-6_14.

Abstract

Transgenic mouse are reliable, convenient models for studying human hepatocellular carcinoma (HCC). The development of a synthetically engineered Sleeping Beauty (SB) transposon system further enables the viral-free, efficient delivery of desired oncogenes to mouse tissues. Here, we describe an SB transposon-based approach to induce HCC in mice by expressing a hyperactive form of N-RAS, N-RASG12V, while silencing the endogenous Trp53 gene via hydrodynamic tail vein injection, a method to rapidly deliver naked plasmids to mouse liver.

Keywords: Hepatocellular carcinoma; Hydrodynamic tail vein injection; Mouse tumor model; N-RAS; Sleeping Beauty; p53.

MeSH terms

  • Animals
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology
  • DNA Transposable Elements / genetics*
  • Disease Models, Animal
  • Female
  • Gene Transfer Techniques
  • Genetic Therapy / methods
  • Hydrodynamics
  • Liver / pathology
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Plasmids / genetics
  • Tail

Substances

  • DNA Transposable Elements