Depletion of protein kinase C induced by an anti HLA class I monoclonal antibody in phytohemagglutinin activated human T cells

Biochem Biophys Res Commun. 1988 May 16;152(3):951-6. doi: 10.1016/s0006-291x(88)80375-9.

Abstract

Anti HLA Class I Monoclonal Antibody depletes Protein Kinase C (PKC) to 20% of control value in PHA activated human T cells. The effect is reversible: in 24 hours the enzymatic activity returns to 58% of control value. Removal of antibody from the culture medium increases the rate of recovery. Implications of this finding for the modulation by HLA Class I antigens of the proliferative response of T cells to lectins are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal*
  • Cells, Cultured
  • HLA Antigens / immunology*
  • Humans
  • Lymphocyte Activation*
  • Phytohemagglutinins / pharmacology*
  • Protein Kinase C / metabolism*
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / enzymology*

Substances

  • Antibodies, Monoclonal
  • HLA Antigens
  • Phytohemagglutinins
  • Protein Kinase C