Genomic surveillance of the Chikungunya Virus (CHIKV) in Northeast Brazil after the first outbreak in 2014

Rev Soc Bras Med Trop. 2020 Jun 22:53:e20190583. doi: 10.1590/0037-8682-0583-2019. eCollection 2020.

Abstract

Introduction: We performed an epidemiological surveillance of the Chikungunya (CHIKV) lineages in Bahia after the 2014 East/Central/South African (ECSA) genotype outbreak.

Methods: Reverse-transcription polymerase chain reaction (RT-PCR), viral isolation, and phylogenetic analyses were conducted on serum samples from 605 patients with CHIKV-like symptoms during 2014-2018.

Results: Of the 605 samples, 167 were CHIKV-positive. Viral isolation was achieved for 20 samples; their phylogenetic analysis (E2 protein) revealed the presence of ECSA lineage and reinforced the phylogenetic relationship between ECSA and Indian Ocean lineages.

Conclusions: The genomic surveillance of CHIKV showed that only ECSA lineage circulated in Bahia since the 2014 outbreak.

MeSH terms

  • Adult
  • Brazil / epidemiology
  • Chikungunya Fever / epidemiology
  • Chikungunya Fever / virology*
  • Chikungunya virus / genetics*
  • Disease Outbreaks
  • Epidemiological Monitoring
  • Female
  • Genome, Viral / genetics*
  • Genotype
  • Humans
  • Male
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction