An optimized workflow to improve reliability of detection of KIAA1549:BRAF fusions from RNA sequencing data

Alexander C Sommerkamp; Sebastian Uhrig; Damian Stichel; Pascal St-Onge; Pengbo Sun; Natalie Jäger; Andreas von Deimling; Felix Sahm; Stefan M Pfister; Andrey Korshunov; Daniel Sinnett; Nada Jabado; Annika K Wefers; David T W Jones

doi:10.1007/s00401-020-02167-1

An optimized workflow to improve reliability of detection of KIAA1549:BRAF fusions from RNA sequencing data

Acta Neuropathol. 2020 Aug;140(2):237-239. doi: 10.1007/s00401-020-02167-1. Epub 2020 May 31.

Authors

Alexander C Sommerkamp^{1

2

3}, Sebastian Uhrig^{4

5}, Damian Stichel^{6

7}, Pascal St-Onge⁸, Pengbo Sun^{1

3

9}, Natalie Jäger^{1

9}, Andreas von Deimling^{6

7}, Felix Sahm^{1

6

7}, Stefan M Pfister^{1

9

10}, Andrey Korshunov^{1

6

7}, Daniel Sinnett^{8

11}, Nada Jabado¹², Annika K Wefers^{1

6

7}, David T W Jones^{13

14}

Affiliations

¹ Hopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
² Pediatric Glioma Research Group, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
³ Faculty of Biosciences, Heidelberg University, Heidelberg, Germany.
⁴ Computational Oncology Group, Molecular Diagnostics Program at the National Center for Tumor Diseases (NCT) and DKFZ, Heidelberg, Germany.
⁵ Division of Applied Bioinformatics, German Cancer Research Center (DKFZ), Heidelberg, Germany.
⁶ Department of Neuropathology, University Hospital Heidelberg, Heidelberg, Germany.
⁷ Clinical Cooperation Unit Neuropathology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
⁸ Division of Hematology-Oncology, Charles-Bruneau Cancer Centre, CHU Sainte-Justine, Montreal, Canada.
⁹ Division of Pediatric Neurooncology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
¹⁰ Department of Pediatric Oncology, Hematology and Immunology, University Hospital Heidelberg, Heidelberg, Germany.
¹¹ Department of Pediatrics, Faculty of Medicine, University of Montreal, Montreal, QC, Canada.
¹² Department of Pediatrics, Faculty of Medicine, McGill University, Montreal, Canada.
¹³ Hopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany. david.jones@kitz-heidelberg.de.
¹⁴ Pediatric Glioma Research Group, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany. david.jones@kitz-heidelberg.de.

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Astrocytoma / genetics*
Humans
Oncogene Proteins, Fusion / analysis*
Sequence Analysis, RNA / methods*
Workflow*

Substances

BRAF-KIAA1549 fusion protein, human
Oncogene Proteins, Fusion