20-hydroxyecdysone (20-HE) stimulates acid phosphatase activity in the fat body of ligated late-last instar larvae. This effect is time dependent and the specific activity of enzyme increases significantly in hormone treated insects. 20-HE also stimulates general protein synthesis. Cycloheximide treatment either in conjunction with 20-HE or after hormone treatment blocks the increase in enzyme activity as well as increase in protein content. However, actinomycin D treatment does not alter the enzyme activity while it blocks the increase in total RNA as well as increase in protein content.