Supramolecular assemblies mimicking neutrophil extracellular traps for MRSE infection control

Zhentao Huang; Ye Liu; Le Wang; Arbab Ali; Qingxin Yao; Xingyu Jiang; Yuan Gao

doi:10.1016/j.biomaterials.2020.120124

Supramolecular assemblies mimicking neutrophil extracellular traps for MRSE infection control

Biomaterials. 2020 Sep:253:120124. doi: 10.1016/j.biomaterials.2020.120124. Epub 2020 May 15.

Authors

Zhentao Huang¹, Ye Liu², Le Wang³, Arbab Ali³, Qingxin Yao⁴, Xingyu Jiang⁵, Yuan Gao⁶

Affiliations

¹ CAS Center for Excellence in Nanoscience, CAS Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
² Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan, 650000, China.
³ CAS Center for Excellence in Nanoscience, CAS Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China.
⁴ CAS Center for Excellence in Nanoscience, CAS Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
⁵ CAS Center for Excellence in Nanoscience, CAS Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: jiang@sustech.edu.cn.
⁶ CAS Center for Excellence in Nanoscience, CAS Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: gaoy@nanoctr.cn.

PMID: 32447104
DOI: 10.1016/j.biomaterials.2020.120124

Abstract

Neutrophil extracellular traps (NETs) stick to bacteria and prevent infections in vivo, whose activation is upon inflammatory stimuli along with the sudden increase of reactive oxygen species (ROS). Nevertheless, the risky over activation in NETosis may result in deleterious outcome. A big challenge in using NETs for therapeutics is to synthesize an artificial system that can function as NETs in vivo. Here, we developed an in vivo supramolecular assembly system to imitate the innate immune process of NETs to inhibit methicillin-resistant staphylococcus epidermidis (MRSE) infection. Our synthesized small molecules undergo oxidation to form supramolecular nanofibers at inflammatory loci. The in situ formed nanofibers network efficiently traps MRSE cells and prevent them from aggressive dissemination. The extended interactions between nanofibers and bacteria directly result in the death of MRSE via the transcriptomes alterations. In clinically relevant models (intraperitoneal infection and catheter implantation), our supramolecular nets show significant antibacterial activity, yielding a three times efficacy comparing to vancomycin. The spontaneous consumption of ROS and the formation of antibacterial networks create a steady negative feedback system to combat bacterial infections.

Keywords: Antimicrobial resistance; Infection; Neutrophil extracellular traps; Reactive oxygen species; Supramolecular self-assembly.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Extracellular Traps*
Infection Control
Methicillin-Resistant Staphylococcus aureus*
Neutrophils
Staphylococcus epidermidis