CRISPR Lights up In Situ Protein Evolution

Evan M Kerek; Christopher R Cromwell; Basil P Hubbard

doi:10.1016/j.chembiol.2020.05.005

CRISPR Lights up In Situ Protein Evolution

Cell Chem Biol. 2020 May 21;27(5):475-478. doi: 10.1016/j.chembiol.2020.05.005.

Authors

Evan M Kerek¹, Christopher R Cromwell¹, Basil P Hubbard²

Affiliations

¹ Department of Pharmacology, University of Alberta, Edmonton, AB T6G 2R7, Canada.
² Department of Pharmacology, University of Alberta, Edmonton, AB T6G 2R7, Canada. Electronic address: bphubbard@ualberta.ca.

PMID: 32442413
DOI: 10.1016/j.chembiol.2020.05.005

Abstract

In this issue of Cell Chemical Biology, Erdogan et al. (2020) describe a new CRISPR/Cas9-based strategy for performing directed evolution of mammalian proteins in situ. Using this technique to select functional mRuby3 variants within lysosomes, they identify mCRISPRed, a fluorescent protein that displays robust stability and activity at low pH.

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MeSH terms

Animals
CRISPR-Cas Systems* / genetics
Clustered Regularly Interspaced Short Palindromic Repeats*
Organelles