ID30A-3 (MASSIF-3) - a beamline for macromolecular crystallography at the ESRF with a small intense beam

David von Stetten; Philippe Carpentier; David Flot; Antonia Beteva; Hugo Caserotto; Fabien Dobias; Matias Guijarro; Thierry Giraud; Mario Lentini; Sean McSweeney; Antoine Royant; Sebastien Petitdemange; Jeremy Sinoir; John Surr; Olof Svensson; Pascal Theveneau; Gordon A Leonard; Christoph Mueller-Dieckmann

doi:10.1107/S1600577520004002

ID30A-3 (MASSIF-3) - a beamline for macromolecular crystallography at the ESRF with a small intense beam

J Synchrotron Radiat. 2020 May 1;27(Pt 3):844-851. doi: 10.1107/S1600577520004002. Epub 2020 Apr 29.

Authors

David von Stetten¹, Philippe Carpentier¹, David Flot¹, Antonia Beteva¹, Hugo Caserotto¹, Fabien Dobias¹, Matias Guijarro¹, Thierry Giraud¹, Mario Lentini¹, Sean McSweeney¹, Antoine Royant¹, Sebastien Petitdemange¹, Jeremy Sinoir², John Surr¹, Olof Svensson¹, Pascal Theveneau¹, Gordon A Leonard¹, Christoph Mueller-Dieckmann¹

Affiliations

¹ European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France.
² EMBL Outstation Grenoble, 71 Avenue des Martyrs, 38000 Grenoble, France.

Abstract

ID30A-3 (or MASSIF-3) is a mini-focus (beam size 18 µm × 14 µm) highly intense (2.0 × 10¹³ photons s^-1), fixed-energy (12.81 keV) beamline for macromolecular crystallography (MX) experiments at the European Synchrotron Radiation Facility (ESRF). MASSIF-3 is one of two fixed-energy beamlines sited on the first branch of the canted undulator setup on the ESRF ID30 port and is equipped with a MD2 micro-diffractometer, a Flex HCD sample changer, and an Eiger X 4M fast hybrid photon-counting detector. MASSIF-3 is recommended for collecting diffraction data from single small crystals (≤15 µm in one dimension) or for experiments using serial methods. The end-station has been in full user operation since December 2014, and here its current characteristics and capabilities are described.

Keywords: ID30A-3; MASSIF-3; automation; high-brilliance; macromolecular crystallography; mini-focus.

open access.