The novel coronavirus (COVID-19) pneumonia with negative detection of viral ribonucleic acid from nasopharyngeal swabs: a case report

BMC Infect Dis. 2020 Apr 30;20(1):317. doi: 10.1186/s12879-020-05045-z.

Abstract

Background: The novel coronavirus disease 2019 (COVID-19) outbreak started in Wuhan, Hubei, China since Dec 2019 and cases of infection have been continuously reported in various countries. It is now clear that the SARS-COV-2 coronavirus is transmissible from human to human. Nucleic acid detection is considered as the gold standard for the diagnosis of COVID-19. In this case report, we describe our experience in detection of SARS-COV-2 from a confirmed patient using nucleic acid test of bronchoalveolar-lavage fluid (BALF) samples but not nasopharyngeal swabs.

Case presentation: We present a case of severely ill SARS-COV-2 infected 46-year-old man with fever, coughing and chest tightness. We performed viral detection using his BALF samples and imaging method (CT) for confirmation. The patient received combination of interferonalfa-1b and ribavirin, lopinavir and ritonavir for antiviral treatment at different stages. Other medication was also given to him in combination for anti-inflammation, intestinal microbial regulation, phlegm elimination, liver protection and pulmonary fibrosis prevention purposes. We provided oxygen supply to him using BIPAP ventilator and high-flow humidification oxygen therapy instrument to facilitate respiration. The patient was cured and discharged.

Conclusion: This case report described an effective supportive medication scheme to treat SARS-COV-2 infected patient and emphasized the necessity of detection of the viral genome using BALF samples and its significance in the diagnosis and prognosis of the disease.

Keywords: Bronchoalveolar-lavage fluid; COVID-19; Coronavirus; Pneumonia; Tracheoscopy.

Publication types

  • Case Reports

MeSH terms

  • Antiviral Agents / therapeutic use
  • Betacoronavirus
  • Bronchoalveolar Lavage Fluid / virology*
  • COVID-19
  • China
  • Coronavirus Infections / diagnosis*
  • Coronavirus Infections / drug therapy
  • Cough / etiology
  • Fever / etiology
  • Humans
  • Lung / diagnostic imaging
  • Male
  • Middle Aged
  • Nasopharynx / virology
  • Pandemics
  • Pneumonia, Viral / diagnosis*
  • Pneumonia, Viral / drug therapy
  • RNA, Viral / isolation & purification*
  • SARS-CoV-2

Substances

  • Antiviral Agents
  • RNA, Viral