In order to solve the problem of inaccuracy of using GC-QTOF-MS combined with NIST14 database in analyzing Ruta graveolens L. essential oil components, systematic isolation and characterization of chemicals from essential oil were carried on. Seventeen compounds which were 3 terpenoids, 8 lipid compounds, 3 alcohol compounds and 3 ketones were separated by preparative GC after dividing the essential oil into 5 groups of fractions by silica gel column. The chemical structures of isolated compounds were identified by GC-QTOF-MS and NMR. Three new in general compounds (50, 53 and 55) were isolated. And proton and carbon spectra of six compounds were provided for the first time. Through separation and structure identification of the compounds, qualitative analysis of total chemical components of the essential oil was increased from 42.0% to 90.3%. This research would be of great significance to application of Ruta graveolens L. essential oil.
Keywords: NMR; Ruta graveolens L; essential oil; preparative GC.