A Chromosome-Scale Genome Assembly Resource for Myriosclerotinia sulcatula Infecting Sedge Grass (Carex sp.)

Mol Plant Microbe Interact. 2020 Jul;33(7):880-883. doi: 10.1094/MPMI-03-20-0060-A. Epub 2020 Jun 1.

Abstract

The fungus Myriosclerotinia sulcatula is a close relative of the notorious polyphagous plant pathogens Botrytis cinerea and Sclerotinia sclerotiorum but exhibits a host range restricted to plants from the Carex genus (Cyperaceae family). To date, there are no genomic resources available for fungi in the Myriosclerotinia genus. Here, we present a chromosome-scale reference genome assembly for M. sulcatula. The assembly contains 24 contigs with a total length of 43.53 Mbp, with scaffold N50 of 2,649.7 kbp and N90 of 1,133.1 kbp. BRAKER-predicted gene models were manually curated using WebApollo, resulting in 11,275 protein-coding genes that we functionally annotated. We provide a high-quality reference genome assembly and annotation for M. sulcatula as a resource for studying evolution and pathogenicity in fungi from the Sclerotiniaceae family.

Keywords: Myriosclerotinia sulcatula; Sclerotinia; fungal pathogen; genome; grass plants; host specific; long read sequencing; proteomics.

MeSH terms

  • Ascomycota* / genetics
  • Ascomycota* / pathogenicity
  • Carex Plant / microbiology*
  • Chromosomes, Fungal / genetics
  • Genome, Fungal*
  • Plant Diseases / microbiology*