Objective: To compare intact dissection and segmented dissection of cochlear surface preparation in adult mice. Methods: From February to March, 2019, Six adult C57BL/6 mice were randomly divided into 2 groups: one group (3 mice) for the intact dissection while the other group (3 mice) for the segmented dissection. Cochlear hair cells were labeled with phalloidin for evaluation of the integrity of the basilar membrane. Results: The basilar membranes can be completely dissected from the cochlea by two approaches. The average dissection time is (16.33±1.86)min with the intact dissection approach while (23.66±3.88) min with the segmented dissection(t=-4.173, P=0.002). Immunofluorescence analysis showed all cochlear hair cells werevisible and intact in two groups. Conclusion: Cochlear basilar membrane can be dissected intact in a short time through both approaches. The approaches selection is dependent on the purpose of experiment and operators' experience.
目的： 对完整与分段取材法解剖小鼠耳蜗基底膜进行比较。 方法： 2019年2—3月，将6只10周龄C57BL/6小鼠以随机数字表法分成2组，分别应用完整取材法及分段取材法进行基底膜取材。应用异硫氰酸荧光素标记的鬼笔环肽荧光染色方法标记耳蜗毛细胞并进行计数，观察基底膜的完整性以及荧光染色效果。SPSS 19.0软件进行统计学分析。 结果： 两种方法均能完整或分段将基底膜完全剥离，完整取材法耗时明显短于分段取材法，差异有统计学意义[（16.33±1.86）min比（23.66±3.88）min，t=-4.173, P=0.002]。鬼笔环肽荧光染色显示两组基底膜毛细胞均保存完整，完整取材法及分段取材法取得的基底膜全长比较差异无统计学意义[（5.92±0.22）mm比（5.72±0.16）mm，t=1.822,P=0.099]。 结论： 应用两种取材方法均可较快分离基底膜并保持其结构的完整性。操作者可根据技术熟练程度及实验目的选择不同的方法。.
Keywords: Cochlea; Hair cells; Mice; Surface preparation.