CircRNA_100876 sponges miR-136 to promote proliferation and metastasis of gastric cancer by upregulating MIEN1 expression

Gene. 2020 Jul 20:748:144678. doi: 10.1016/j.gene.2020.144678. Epub 2020 Apr 17.

Abstract

Previous reports have revealed that circRNA_100876 was extremely important in the progression of triple-negative breast cancer. Nevertheless, the mechanism towards the role of circRNA_100876 in Gastric cancer (GC) remains unknown. Here, we determined circRNA_100876 expression by quantitative real-time PCR (qRT-PCR) in twenty pairs of GC tissues and adjacent tissues. Our data indicated that the expression of circRNA_100876 was raised in GC tissues. In vitro, functional experiments confirmed that cell proliferation, invasion along with migration was promoted by circRNA_100876 in GC tissues. Simultaneously, relative luciferase assay uncovered that circRNA_100876 functioned as a sponge for miR-136, followed by retarding miR-136-induced inhibited effects on the corresponding target, MIEN1. Moreover, we revealed that the expression of MIEN1 was up-regulated and correlated to much worse prognosis of GC. Collectively, our data identified that the promotion of GC growth and metastasis induced by circRNA_100876 interacted with miR-136 and MIEN1, indicating an emerging announcement for uncovering the potential mechanism of GC progression.

Keywords: Cell proliferation; Invasion; MIEN1; Migration; circRNA_100876; miRNA sponge.

MeSH terms

  • Biomarkers, Tumor / metabolism
  • Cell Line, Tumor
  • Cell Proliferation / genetics*
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics*
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics
  • Neoplasm Metastasis / genetics*
  • Neoplasm Proteins / genetics*
  • Prognosis
  • RNA, Circular / metabolism*
  • RNA, Circular / physiology
  • Real-Time Polymerase Chain Reaction
  • Stomach Neoplasms / pathology*
  • Up-Regulation / genetics*

Substances

  • Biomarkers, Tumor
  • Intracellular Signaling Peptides and Proteins
  • MIEN1 protein, human
  • MIRN136 microRNA, human
  • MicroRNAs
  • Neoplasm Proteins
  • RNA, Circular