Understanding the sheet size-antibacterial activity relationship of graphene oxide and the nano-bio interaction-based physical mechanisms

Colloids Surf B Biointerfaces. 2020 Jul:191:111009. doi: 10.1016/j.colsurfb.2020.111009. Epub 2020 Apr 12.

Abstract

The antibiotics-independent antimicrobial activity of graphene oxide (GO) is of great importance since antibiotic therapy is facing great challenges from drug resistance. However, the relations of GO size with its antimicrobial activity and how the size regulates the antibacterial mechanisms are still unknown. Herein, we fabricated four GO suspensions with different sizes and demonstrated the parabolic relationship between GO size and its antibacterial activity against the Gram-positive cariogenic bacterium Streptococcus mutans. More interestingly, we found out how GO size regulated the nano-bio interaction-based physical antibacterial mechanisms. Increasing the size reduced the cutting effect but enhanced the cell entrapment effect, and vice versa. In conclusion, GO size affects its edge density and lateral dimension, further regulates its physical antibacterial mechanisms in different orientations and ultimately determines its activity. These findings provide a deep understanding of GO antibacterial property and may guide the design and development of GO for clinical use.

Keywords: Antimicrobial; Bacteria; Dental caries; Graphene; Infection; Nanomaterials.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Graphite / chemistry
  • Graphite / pharmacology*
  • Microbial Sensitivity Tests
  • Nanoparticles / chemistry*
  • Particle Size
  • Streptococcus mutans / drug effects*
  • Surface Properties

Substances

  • Anti-Bacterial Agents
  • graphene oxide
  • Graphite