Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
Keywords: 2019 novel coronavirus disease; COVID-19; SARS; SARS-CoV-2; coronavirus; coronavirus disease; molecular detection; rRT-PCR; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.