Production of low-calorie structured lipids from spent coffee grounds or olive pomace crude oils catalyzed by immobilized lipase in magnetic nanoparticles

Danyelle A Mota; Devi Rajan; Giuditta C Heinzl; Natália M Osório; Jorge Gominho; Laiza C Krause; Cleide M F Soares; K Madhavan Nampoothiri; Rajeev K Sukumaran; Suzana Ferreira-Dias

doi:10.1016/j.biortech.2020.123223

Production of low-calorie structured lipids from spent coffee grounds or olive pomace crude oils catalyzed by immobilized lipase in magnetic nanoparticles

Bioresour Technol. 2020 Jul:307:123223. doi: 10.1016/j.biortech.2020.123223. Epub 2020 Mar 19.

Authors

Affiliations

¹ Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Linking Landscape, Environment, Agriculture and Food, Lisbon, Portugal; Institute of Technology and Research (ITP), Avenida Murilo Dantas 300 - Farolândia, Aracaju, Brazil; Tiradentes University (UNIT), Avenida Murilo Dantas 300 - Farolândia, Aracaju, Brazil.
² Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Linking Landscape, Environment, Agriculture and Food, Lisbon, Portugal; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala, India.
³ Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Linking Landscape, Environment, Agriculture and Food, Lisbon, Portugal.
⁴ Instituto Politécnico de Setúbal, Escola Superior de Tecnologia do Barreiro, Lavradio, Portugal.
⁵ Instituto Superior de Agronomia, Universidade de Lisboa, Centro de Estudos Florestais, Lisbon, Portugal.
⁶ Institute of Technology and Research (ITP), Avenida Murilo Dantas 300 - Farolândia, Aracaju, Brazil; Tiradentes University (UNIT), Avenida Murilo Dantas 300 - Farolândia, Aracaju, Brazil.
⁷ Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala, India.
⁸ Instituto Superior de Agronomia, Universidade de Lisboa, LEAF, Linking Landscape, Environment, Agriculture and Food, Lisbon, Portugal. Electronic address: suzanafdias@mail.telepac.pt.

PMID: 32220818
DOI: 10.1016/j.biortech.2020.123223

Abstract

In this study, crude oils extracted from spent coffee grounds (SCG) and olive pomace (OP) were used as raw-material to synthesize low-calorie triacylglycerols, either by acidolysis with capric acid, or by interesterification with ethyl caprate, in solvent-free media, catalyzed by sn-1,3 regioselective lipases. The Rhizopus oryzae lipase (ROL) was immobilized in magnetite nanoparticles (MNP-ROL) and tested as novel biocatalyst. MNP-ROL performance was compared with that of the commercial immobilized Thermomyces lanuginosus lipase (Lipozyme TL IM). For both oils, Lipozyme TL IM preferred interesterification over acidolysis. MNP-ROL catalyzed reactions were faster and acidolysis was preferred with yields of c.a. 50% new triacylglycerols after 3 h acidolysis of OP or SCG oils. MNP-ROL was very stable following the Sadana deactivation model with half-lives of 163 h and 220 h when reused in batch acidolysis and interesterification of OP oil, respectively.

Keywords: Lipase; Magnetic nanoparticles; Olive pomace oil; Spent coffee grounds oil; Structured lipids.

MeSH terms

Catalysis
Coffee
Enzymes, Immobilized
Esterification
Lipase
Lipids
Magnetite Nanoparticles*
Olive Oil
Petroleum*

Substances

Coffee
Enzymes, Immobilized
Lipids
Magnetite Nanoparticles
Olive Oil
Petroleum
Lipase