Worry and FRET: ROS Production Leads to Fluorochrome Tandem Degradation and impairs Interpretation of Flow Cytometric Results

Immunity. 2020 Mar 17;52(3):419-421. doi: 10.1016/j.immuni.2020.02.003.
No abstract available

Publication types

  • Letter
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Benzothiazoles / chemistry
  • Benzothiazoles / metabolism
  • Carbocyanines / chemistry
  • Carbocyanines / metabolism
  • Flow Cytometry / methods*
  • Fluorescence Resonance Energy Transfer / methods*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Granulocytes / chemistry
  • Granulocytes / metabolism
  • Humans
  • Mercaptoethanol / chemistry
  • Mercaptoethanol / metabolism
  • Phycocyanin / chemistry
  • Phycocyanin / metabolism
  • Reactive Oxygen Species / metabolism*
  • Reproducibility of Results

Substances

  • Benzothiazoles
  • Carbocyanines
  • Fluorescent Dyes
  • Reactive Oxygen Species
  • allophycocyanin
  • Phycocyanin
  • cyanine dye 7
  • Mercaptoethanol