Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin

Nat Commun. 2020 Mar 9;11(1):1313. doi: 10.1038/s41467-020-15041-1.

Abstract

Erythropoietin (EPO), named after its role in hematopoiesis, is also expressed in mammalian brain. In clinical settings, recombinant EPO treatment has revealed a remarkable improvement of cognition, but underlying mechanisms have remained obscure. Here, we show with a novel line of reporter mice that cognitive challenge induces local/endogenous hypoxia in hippocampal pyramidal neurons, hence enhancing expression of EPO and EPO receptor (EPOR). High-dose EPO administration, amplifying auto/paracrine EPO/EPOR signaling, prompts the emergence of new CA1 neurons and enhanced dendritic spine densities. Single-cell sequencing reveals rapid increase in newly differentiating neurons. Importantly, improved performance on complex running wheels after EPO is imitated by exposure to mild exogenous/inspiratory hypoxia. All these effects depend on neuronal expression of the Epor gene. This suggests a model of neuroplasticity in form of a fundamental regulatory circle, in which neuronal networks-challenged by cognitive tasks-drift into transient hypoxia, thereby triggering neuronal EPO/EPOR expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • Brain / physiopathology*
  • Cell Differentiation / drug effects
  • Cognition / drug effects
  • Dendritic Spines / drug effects
  • Dendritic Spines / metabolism
  • Erythropoietin / metabolism*
  • Erythropoietin / pharmacology
  • Female
  • Gene Deletion
  • Humans
  • Hypoxia / metabolism*
  • Hypoxia / physiopathology*
  • Male
  • Mice, Inbred C57BL
  • Models, Neurological
  • Motor Activity / drug effects
  • Neurogenesis* / drug effects
  • Neuronal Plasticity* / drug effects
  • Physical Conditioning, Animal
  • Physical Endurance / drug effects
  • Proto-Oncogene Proteins c-fos / metabolism
  • Pyramidal Cells / drug effects
  • Pyramidal Cells / metabolism
  • Receptors, Erythropoietin / metabolism
  • Transcriptome / drug effects
  • Transcriptome / genetics

Substances

  • Proto-Oncogene Proteins c-fos
  • Receptors, Erythropoietin
  • Erythropoietin