Genomic profiling of multiple myeloma: New insights and modern technologies

Best Pract Res Clin Haematol. 2020 Mar;33(1):101153. doi: 10.1016/j.beha.2020.101153. Epub 2020 Jan 27.

Abstract

Advances in technologies for genomic profiling, primarily with next generation sequencing, have lead to a better understanding of the complex genomic landscape in multiple myeloma. Integrated analysis of whole genome, exome and transcriptome sequencing has lead to new insights on disease drivers including translocations, copy number alterations, somatic mutations, and altered gene expression. Disease progression in multiple myeloma is largely driven by structural variations including the traditional immunoglobulin heavy chain (IGH) translocations and hyperdiploidy which are early events in myelomagenesis as well as more complex events spanning over multiple chromosomes and involving amplifications and deletions. In this review, we will discuss recent insights on the genomic landscape of multiple myeloma and their implications for disease progression and personalized treatment. We will review how sequencing assays compare to current clinical methods and give an overview of modern technologies for interrogating genomic aberrations.

Keywords: Copy number alteration; Genomics; Multiple myeloma; Somatic mutations; Structural variation.

Publication types

  • Review

MeSH terms

  • Bone Marrow / immunology
  • Bone Marrow / pathology
  • DNA Copy Number Variations
  • DNA, Neoplasm / genetics*
  • DNA, Neoplasm / immunology
  • Disease Progression
  • Genome, Human
  • Genomics / methods*
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin Heavy Chains / immunology
  • Multiple Myeloma / classification
  • Multiple Myeloma / diagnosis*
  • Multiple Myeloma / genetics
  • Multiple Myeloma / immunology
  • Mutation
  • Neoplasm, Residual
  • Plasma Cells / immunology
  • Plasma Cells / pathology
  • Translocation, Genetic*

Substances

  • DNA, Neoplasm
  • Immunoglobulin Heavy Chains