Optical Microscopy and Coherence Tomography of Cancer in Living Subjects

Peng Si; Alexander Honkala; Adam de la Zerda; Bryan Ronain Smith

doi:10.1016/j.trecan.2020.01.008

Optical Microscopy and Coherence Tomography of Cancer in Living Subjects

Trends Cancer. 2020 Mar;6(3):205-222. doi: 10.1016/j.trecan.2020.01.008. Epub 2020 Feb 5.

Authors

Peng Si¹, Alexander Honkala², Adam de la Zerda³, Bryan Ronain Smith⁴

Affiliations

¹ Department of Structural Biology, Stanford University, Stanford, CA 94305, USA; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USA.
² Department of Radiation Oncology, Stanford University, Stanford, CA 94305, USA.
³ Department of Structural Biology, Stanford University, Stanford, CA 94305, USA; Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USA; Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA; The Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: adlz@stanford.edu.
⁴ Molecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305, USA; Department of Biomedical Engineering and Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI 48824, USA. Electronic address: smit2901@msu.edu.

PMID: 32101724
DOI: 10.1016/j.trecan.2020.01.008

Abstract

Intravital microscopy (IVM) and optical coherency tomography (OCT) are two powerful optical imaging tools that allow visualization of dynamic biological activities in living subjects with subcellular resolutions. Recent advances in labeling and label-free techniques empower IVM and OCT for a wide range of preclinical and clinical cancer imaging, providing profound insights into the complex physiological, cellular, and molecular behaviors of tumors. Preclinical IVM and OCT have elucidated many otherwise inscrutable aspects of cancer biology, while clinical applications of IVM and OCT are revolutionizing cancer diagnosis and therapies. We review important progress in the fields of IVM and OCT for cancer imaging in living subjects, highlighting key technological developments and their emerging applications in fundamental cancer biology research and clinical oncology investigation.

Keywords: clinical oncology; contrast agents; in vivo cancer imaging; intravital microscopy; living subjects; optical coherence tomography; tumor microenvironment.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Animals
Endoscopy / methods
Fluorescent Dyes
Fourier Analysis
Humans
Intravital Microscopy / methods*
Lymphangiogenesis
Lymphocytes, Tumor-Infiltrating
Microscopy, Fluorescence / methods
Microscopy, Interference / methods
Neoplasms / blood supply
Neoplasms / diagnostic imaging*
Neoplasms / immunology
Neoplasms, Experimental / diagnostic imaging
Neovascularization, Pathologic / diagnostic imaging
Tomography, Optical Coherence / methods*
Tumor Microenvironment
Tumor-Associated Macrophages

Substances

Fluorescent Dyes

Grants and funding

18TPA34230113/AHA_/American Heart Association-American Stroke Association/United States