Current advances in microcell-mediated chromosome transfer technology and its applications

Teruhiko Suzuki; Yasuhiro Kazuki; Takahiko Hara; Mitsuo Oshimura

doi:10.1016/j.yexcr.2020.111915

Current advances in microcell-mediated chromosome transfer technology and its applications

Exp Cell Res. 2020 May 1;390(1):111915. doi: 10.1016/j.yexcr.2020.111915. Epub 2020 Feb 21.

Authors

Teruhiko Suzuki¹, Yasuhiro Kazuki², Takahiko Hara³, Mitsuo Oshimura⁴

Affiliations

¹ Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
² Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan; Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
³ Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan; Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan; Graduate School of Science, Department of Biological Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo, 192-0397, Japan.
⁴ Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan; Trans Chromosomics Inc., 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. Electronic address: moshimura@trans-chromo.com.

PMID: 32092294
DOI: 10.1016/j.yexcr.2020.111915

Abstract

Chromosomes and chromosomal gene delivery vectors, human/mouse artificial chromosomes (HACs/MACs), can introduce megabase-order DNA sequences into target cells and are used for applications including gene mapping, gene expression control, gene/cell therapy, and the development of humanized animals and animal models of human disease. Microcell-mediated chromosome transfer (MMCT), which enables chromosome transfer from donor cells to target cells, is a key technology for these applications. In this review, we summarize the principles of gene transfer with HACs/MACs; their engineering, characteristics, and utility; and recent advances in the chromosome transfer technology.

Keywords: Chromosome; Chromosome engineering; HAC/MAC; MMCT; Microcell-mediated chromosome transfer.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Chromosome Mapping / methods
Chromosomes, Artificial, Mammalian / genetics*
Gene Transfer Techniques*
Genetic Engineering / methods*
Humans