Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase

Ravil R Garafutdinov; Assol R Sakhabutdinova; Maxim S Kupryushkin; Dmitrii V Pyshnyi

doi:10.1016/j.dib.2020.105188

Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase

Data Brief. 2020 Jan 25:29:105188. doi: 10.1016/j.dib.2020.105188. eCollection 2020 Apr.

Authors

Ravil R Garafutdinov¹, Assol R Sakhabutdinova¹, Maxim S Kupryushkin², Dmitrii V Pyshnyi²

Affiliations

¹ Institute of Biochemistry and Genetics, Ufa Federal Research Center of the Russian Academy of Sciences, Russian Federation.
² Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Russian Federation.

Abstract

This article reports experimental data related to the research article entitled "Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase" (R.R. Garafutdinov, A.R. Sakhabutdinova, M.S. Kupryushkin, D.V. Pyshnyi, 2020) [1]. Here, multimerization efficiency in terms of Tt (time-to-threshold) values obtained for artificial DNA templates with the different nucleotide sequences during isothermal amplification with Bst exo- DNA polymerase is given. Data on the influence of phosphoryl guanidine primers (PGO) on multimerization for the LTc template which has shown high efficiency of multimerization are presented as well.

Keywords: Bst exo- DNA polymerase; Isothermal amplification; Multimerization; Phosphoryl guanidine oligonucleotides (PGO).