Dual-Wavelength Electrochemiluminescence Ratiometric Biosensor for NF-κB p50 Detection with Dimethylthiodiaminoterephthalate Fluorophore and Self-Assembled DNA Tetrahedron Nanostructures Probe

ACS Appl Mater Interfaces. 2020 Mar 11;12(10):11409-11418. doi: 10.1021/acsami.0c01243. Epub 2020 Mar 2.

Abstract

In this work, we fabricated a dual-wavelength electrochemiluminescence ratiometric biosensor based on electrochemiluminescent resonance energy transfer (ECL-RET). In this biosensor, Au nanoparticle-loaded graphitic phase carbon nitride (Au-g-C3N4) as a donor and Au-modified dimethylthiodiaminoterephthalate (TAT) analogue (Au@TAT) as an acceptor were investigated for the first time. Besides, tetrahedron DNA probe was immobilized onto Au-g-C3N4 to improve the binding efficiency of the transcription factor and ECL ratiometric changes on the basis of the ratio of ECL intensities at 595 and 460 nm, which were obtained through the formation of a sandwich structure of DNA probe-antigen-antibody. Our biosensor achieved the assay of NF-κB p50 with a detection limit of 5.8 pM as well as high stability and specificity.

Keywords: DNA tetrahedron nanostructures; NF-κB p50; dimethylthiodiaminoterephthalate (TAT); electrochemiluminescence; ratiometric biosensor.

MeSH terms

  • Biosensing Techniques / methods*
  • DNA / chemistry*
  • Electrochemical Techniques / methods*
  • Fluorescent Dyes / chemistry
  • Humans
  • Molecular Probes / chemistry
  • NF-kappa B p50 Subunit / analysis*
  • Nanostructures / chemistry*
  • Phthalic Acids / chemistry

Substances

  • Fluorescent Dyes
  • Molecular Probes
  • NF-kappa B p50 Subunit
  • Phthalic Acids
  • DNA