Fluorescence resonance energy transfer (FRET)-based nanoarchitecture for monitoring deubiquitinating enzyme activity

Chem Commun (Camb). 2020 Mar 14;56(21):3183-3186. doi: 10.1039/c9cc09808a. Epub 2020 Feb 18.

Abstract

A novel nanoarchitecture (MSN-Tb-UbR) was prepared by modifying rhodamine B-labelled Ubs (Ub-Rs) on the surface of mesoporous silica nanoparticles (MSNs) loaded with Tb3+-complexes. The MSN-Tb-UbR exhibits ratiometric sensing ability for DUB (UCH-L1) with good sensitivity and selectivity.

MeSH terms

  • Fluorescence Resonance Energy Transfer / methods
  • Fluorescent Dyes / chemistry
  • Nanoparticles / chemistry*
  • Porosity
  • Rhodamines / chemistry
  • Silicon Dioxide / chemistry*
  • Surface Properties
  • Terbium / chemistry
  • Ubiquitin Thiolesterase / analysis*

Substances

  • Fluorescent Dyes
  • Rhodamines
  • Terbium
  • Silicon Dioxide
  • Ubiquitin Thiolesterase
  • rhodamine B