Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human

Yuan Gao; Yanlin Wang; Zhuoya Wang; Huifang Sun; Rui Zhang; Jing Yang; Yutao Liu; Han Liu; Qi Zhang; Shoutao Zhang; Changhe Shi; Yuming Xu; Bo Song

doi:10.1016/j.scr.2020.101717

Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human

Stem Cell Res. 2020 Mar:43:101717. doi: 10.1016/j.scr.2020.101717. Epub 2020 Jan 25.

Authors

Yuan Gao¹, Yanlin Wang¹, Zhuoya Wang¹, Huifang Sun¹, Rui Zhang¹, Jing Yang¹, Yutao Liu¹, Han Liu¹, Qi Zhang¹, Shoutao Zhang², Changhe Shi¹, Yuming Xu³, Bo Song⁴

Affiliations

¹ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
² School of life sciences, Zhengzhou University, Zhengzhou, Henan 450001, China.
³ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: xuyuming@zzu.edu.cn.
⁴ Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: songbo76@sina.com.

PMID: 32007761
DOI: 10.1016/j.scr.2020.101717

Abstract

Dermal fibroblasts obtained from a 43-year-old healthy man were successfully transformed into induced pluripotent stem cells (iPSCs) by employing episomal plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and l-MYC. The iPSCs showed a normal karyotype and exhibited the potential to differentiate into three germ layers in a teratoma assay, which is often used to assess the pluripotency of stem cells. This iPSC line may be subsequently used for drug screens, biological tissue engineering, and cell transplantations.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Fibroblasts / metabolism*
Healthy Volunteers
Humans
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4
Male