OXA-181-Producing Extraintestinal Pathogenic Escherichia coli Sequence Type 410 Isolated from a Dog in Portugal

Michael Brilhante; Juliana Menezes; Adriana Belas; Claudia Feudi; Stefan Schwarz; Constança Pomba; Vincent Perreten

doi:10.1128/AAC.02298-19

OXA-181-Producing Extraintestinal Pathogenic Escherichia coli Sequence Type 410 Isolated from a Dog in Portugal

Antimicrob Agents Chemother. 2020 Mar 24;64(4):e02298-19. doi: 10.1128/AAC.02298-19. Print 2020 Mar 24.

Authors

Michael Brilhante^{1

2}, Juliana Menezes³, Adriana Belas³, Claudia Feudi⁴, Stefan Schwarz⁴, Constança Pomba³, Vincent Perreten⁵

Affiliations

¹ Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
² Graduate School of Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
³ Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
⁴ Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
⁵ Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland vincent.perreten@vetsuisse.unibe.ch.

Abstract

Two multidrug-resistant and carbapenemase-producing Escherichia coli clones of sequence type 410 were isolated from fecal samples of a dog with skin infection on admission to an animal hospital in Portugal and 1 month after discharge. Whole-genome sequencing revealed a 126,409-bp Col156/IncFIA/IncFII multidrug resistance plasmid and a 51,479-bp IncX3 bla_OXA-181-containing plasmid. The chromosome and plasmids carried virulence genes characteristic for uropathogenic E. coli, indicating that dogs may carry multidrug-resistant E. coli isolates related to those causing urinary tract infections in humans.

Keywords: Escherichia coli; ExPEC; carbapenemase; companion animals; pets; veterinary.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / metabolism
Cat Diseases / microbiology
Cats
Dog Diseases / microbiology*
Dogs
Escherichia coli Infections / microbiology
Escherichia coli Infections / veterinary*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Extraintestinal Pathogenic Escherichia coli / genetics*
Extraintestinal Pathogenic Escherichia coli / isolation & purification*
Extraintestinal Pathogenic Escherichia coli / metabolism
Extraintestinal Pathogenic Escherichia coli / pathogenicity
Feces / microbiology
Gastrointestinal Microbiome
Genome, Bacterial
Phylogeny
Plasmids
Portugal
Skin Diseases, Bacterial / microbiology
Skin Diseases, Bacterial / veterinary
Urinary Tract Infections / microbiology
Urinary Tract Infections / veterinary
Virulence Factors / genetics
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Bacterial Proteins
Escherichia coli Proteins
Virulence Factors
beta-Lactamases
beta-lactamase OXA-181, E coli
carbapenemase