The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies

Cell Adh Migr. 2020 Dec;14(1):12-23. doi: 10.1080/19336918.2020.1713532.

Abstract

In this study, we aimed to study the effect of fluid shear stress on fibroblasts and BMSCs on plane and groove topographies. The results showed that 0.6-Hz stress had the greatest influence on the alignment, polarity, migration and adhesion of fibroblasts on plane by increasing the expression of reoriented actin and vinculin; whereas 1.0-Hz stress promoted differentiation of fibroblasts into myofibroblasts by increasing Col-I and α-SMA expression. Interestingly, under the given frequency stress, the groove structure strengthened the above characteristics of fibroblasts beyond adhesion, and promoted differentiation of BMSCs into myofibroblasts. The above results indicate that 0.6 Hz may improve the implant-tissue sealing, while 1.0-Hz stress probably causes the disordered fiber deposition around implants.

Keywords: Frequency; adhesion; alignment; differentiate; migration; polarity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Differentiation
  • Cell Shape
  • Fibroblasts / cytology*
  • Fibroblasts / ultrastructure
  • Fluorescence
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism
  • Mice
  • NIH 3T3 Cells
  • Shear Strength*
  • Stress, Mechanical*

Grants and funding

The authors are grateful for the financial supports from the National Key R&D Program of China (grant No. 2016YFC1100300), the National Natural Science Foundation of China (grant No. 81672189 and 81430049), the Shandong Provincial Natural Science Foundation (grant No. ZR2014HP027), the Linyi Municipal Science and Technology Development Plan (grant No. 201818015).