Abstract
Nucleotide exchange factors (NEFs) play an important role in plant abiotic stress response, but their characteristics and functions in seagrass have not been studied. Zostera japonica (Z. japonica) is one of the most widely distributed seagrass species in China and are distributed in subtropical and temperate coastal areas. Z. japonica is intertidal seagrass, which often undergoes heat stress during summer when the tide ebbs. Overexpression of ZjFes1 in Arabidopsis results in an increase in heat tolerance. We found that ZjFes1 associates with ZjHsp70 in vivo by yeast two-hybrid and bimolecular fluorescence complementarity (BiFC). Overexpression of ZjFes1 leads to selective reduction of Hsp70 transcription and an increase in Hsp101. In conclusion, our results suggest that ZjFes1 may be an active regulator of heat tolerance.
Keywords:
Seagrass; nucleotide exchange factor; thermotelerance.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Arabidopsis / genetics
-
Arabidopsis / metabolism*
-
HSP70 Heat-Shock Proteins / genetics
-
HSP70 Heat-Shock Proteins / metabolism
-
Heat-Shock Response
-
Plant Proteins / genetics
-
Plant Proteins / metabolism
-
Plants, Genetically Modified / genetics
-
Plants, Genetically Modified / metabolism*
-
Transcription Factors / genetics
-
Transcription Factors / metabolism
-
Zosteraceae / genetics
-
Zosteraceae / metabolism
Substances
-
HSP101 protein, plant
-
HSP70 Heat-Shock Proteins
-
Plant Proteins
-
Transcription Factors
Grants and funding
This work was supported by the Natural Science Foundation of Guangxi Province [2018GXNSFBA281062], the Basic scientific foundation of Guangxi institute of public welfare scientific reaearch [2018GMRC02], the Guangxi Key Lab of Mangrove Conservation and Utilization funds [GKLMC-16A01] and the Distinguished Employment offered Unit of Guangxi for Conservation and Ecological Monitoring of Mangroves and Seagrasses.