Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes

Kai Ji; Yongchang Xu; Jian Sun; Man Huang; Xu Jia; Chengjian Jiang; Youjun Feng

doi:10.1080/21505594.2019.1706913

Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes

Virulence. 2020 Dec;11(1):49-56. doi: 10.1080/21505594.2019.1706913.

Authors

Kai Ji^{1

2}, Yongchang Xu^{2

3}, Jian Sun⁴, Man Huang², Xu Jia³, Chengjian Jiang^{1

5}, Youjun Feng^{1

2

3

4}

Affiliations

¹ Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, Guangxi, China.
² Department of Pathogen Biology & Microbiology and Department of General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
³ Non-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical College, Chengdu, Sichuan, China.
⁴ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
⁵ Guangxi Key Laboratory of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Guangxi, China.

Abstract

A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of tet(X) variants. This method is successfully applied in the screen and validation of tet(X) genes in the field and clinic bacterial samples. In addition, we found that the formerly proposed tet(X1) is a premature truncated version by the inappropriate annotation, and fixed this error. Overall, it might be the first genetic tool for the detection of different Tet(X) members.

Keywords: Antimicrobial resistance; Multiplex PCR; Tet(X); Tet(X1); Tet(X2); Tet(X3); Tet(X4); Tet(X5); Tigecycline resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / genetics
Bacteria / isolation & purification
Bacterial Proteins / genetics
Genes, Bacterial / genetics*
Multiplex Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Tetracycline Resistance / genetics*
Tigecycline

Substances

Bacterial Proteins
Tet M resistance protein, Bacteria
Tigecycline

Grants and funding

This work was supported by the National Natural Science Foundation of China [31830001, 81772142 & 31570027, for YF; 31760437, for CJ] and National Key R&D Program of China [2017YFD0500202]. Dr. Feng is a recipient of the national “Young 1000 Talents” Award of China.