A novel and sensitive ratiometric fluorescence assay for carbendazim based on N-doped carbon quantum dots and gold nanocluster nanohybrid

Abstract

A novel fluorescence "turn on" ratiometric fluorescent sensor was employed to determine carbendazim. The sensing process was achieved through the strong fluorescence resonance energy transfer (FRET) between nitrogen doped carbon quantum dots (N-CQDs) and gold nanocluster (AuNCs). The photoluminescence intensity of N-CQDs can be deactivated by AuNCs through FRET effect and recovered by the addition of carbendazim. The ratiometric detection of carbendazim is achieved by recording the photoluminescence and second-order Rayleigh scattering (SRS) signal of N-CQDs/AuNCs system. With the introduction of carbendazim to the sensing platform resulted in the photoluminescence and SRS signal of N-CQDS/AuNCs enhancing. UV-vis absorption, Zeta potential and fluorescence lifetime analyses indicate that the fluorescence turn on process can be attributed to the aggregation of AuNCs breaks the FRET process and increases SRS intensity. N-CQDs/AuNCs probe present a good sensitivity and selectivity for carbendazim detection, with two linear response ranges (1-100 μM, 150-1000 μM), low detection limit of 0.83 μM and 37.25 μM. Furthermore, real sample analyses indicate that the as-presented sensor has potentials in carbendazim determination in real sample analyses.

Keywords: Au nanocluster; Fluorescence resonance energy transfer; N doped carbon quantum dots; Ratiometric fluorescent sensor.