An enzyme-initiated DNAzyme motor for RNase H activity imaging in living cell

Chem Commun (Camb). 2020 Jan 14;56(4):639-642. doi: 10.1039/c9cc08692g. Epub 2019 Dec 13.

Authors

Namin Hu¹, Yafang Wang¹, Chang Liu¹, Manman He¹, Cunpeng Nie¹, Juan Zhang¹, Qiaoqin Yu¹, Chuan Zhao¹, Tingting Chen¹, Xia Chu¹

Affiliation

¹ Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. chenting1104@hnu.edu.cn xiachu@hnu.edu.cn.

PMID: 31833487
DOI: 10.1039/c9cc08692g

Abstract

In this work, we have developed a novel DNAzyme motor initiated by endogenous enzyme for sensitive imaging of intracellular RNase H activity.

MeSH terms

Cell Survival
DNA, Catalytic / metabolism*
HeLa Cells
Hep G2 Cells
Humans
MCF-7 Cells
Microscopy, Confocal
Microscopy, Fluorescence
Optical Imaging*
Ribonuclease H / metabolism*

Substances

DNA, Catalytic
Ribonuclease H