Accelerated DNAzyme-based fluorescent nanoprobe for highly sensitive microRNA detection in live cells

Chem Commun (Camb). 2020 Jan 2;56(3):470-473. doi: 10.1039/c9cc08598j.

Abstract

By assembling DNAzyme on DNA nanowires through DNA hybridization, we have developed a novel accelerated DNAzyme-based fluorescent nanoprobe for fast, sensitive and selective detection of miRNA. Moreover, the strategy was successfully applied for in situ imaging of miRNA-21 in different cell lines.

MeSH terms

  • Cell Line, Tumor
  • DNA, Catalytic / chemistry*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Limit of Detection
  • MicroRNAs / analysis*
  • MicroRNAs / metabolism
  • Microscopy, Confocal*
  • Nanowires / chemistry*
  • Nucleic Acid Hybridization
  • Spectrometry, Fluorescence

Substances

  • DNA, Catalytic
  • Fluorescent Dyes
  • MicroRNAs