The protocol allows to define and characterize mitosis distribution patterns in the plant root meristem. The method does not require genetic markers, which makes it applicable to plants of different non-transgenic genotypes, including ecotypes, mutants, and non-model plant species. Computer analysis of the mitosis distribution in three dimensions with iRoCS Toolbox identifies statistically significant changes in proliferation activity within specific root tissues and cell lineages.
Keywords: Arabidopsis; Cell cycle; Cortex; Endodermis; Epidermis; Image analysis; Pericycle; Proliferation; Protophloem; Protoxylem; RAM.