Is it Time to Say Goodbye to Culture and Sensitivity? The Case for Culture-independent Urology

Urology. 2020 Feb:136:112-118. doi: 10.1016/j.urology.2019.11.030. Epub 2019 Nov 29.

Abstract

Next-generation sequencing has highlighted the limitations of conventional culture methods in the role of urology while discovering the intricate details of the role of microbiota in urologic health and disease. This review article explores: the utility and limitations of conventional culture methods; how culture-independent technologies are revolutionizing medicine; and how the implementation of these technologies may lead to improved patient outcomes. Finally, this article discusses the barriers to widespread adoption of culture-independent technologies, with suggestions for how these hurdles may be overcome.

Publication types

  • Review

MeSH terms

  • Bacteriological Techniques*
  • High-Throughput Nucleotide Sequencing*
  • Humans
  • Sensitivity and Specificity
  • Urinary Tract Infections / microbiology*