The 3-hydroxy-3-methylglutaryl-coenzyme A reductase 5 gene from Malus domestica enhances oxidative stress tolerance in Arabidopsis thaliana

Abstract

3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) is the first rate-limiting enzyme regulating the synthesis of terpenoids upstream of the mevalonate (MVA) pathway. In higher plants, members of the HMGR genes families play an important role in plant growth and development and in response to various environmental stresses. In the present study, a novel HMGR gene, designated MdHMGR5, was isolated from apple (Malus domestica L.) and characterized. Expression of MdHMGR5 enhanced the activity of HMGR enzyme in transgenic Arabidopsis thaliana L. plants. Under oxidative stress, transgenic A. thaliana plants over-expressing MdHMGR5 had a higher germination rate, a longer main root length, higher chlorophyll and proline content, and higher activities of antioxidant enzymes. On the other hand, malondialdehyde (MDA) content, relative conductivity and reactive oxygen species (ROS) production rate were significantly lower than in wild type plants. These results indicated that over-expression of MdHMGR5 enhanced plant tolerance to oxidative stress by scavenging ROS in transgenic plants. Over-expression of MdHMGR5 also affected the expression levels of genes in mevalonic acid and 2C-methyl-D-erythritol 4-phosphate (MVA and MEP) pathways of A. thaliana plants. These results indicate that over-expression of MdHMGR5 enhances tolerance to oxidative stress by maintaining photosynthesis and scavenging ROS in transgenic A. thaliana plants.

Keywords: Antioxidant system; HMGR; Oxidative tolerance; Transgenic Arabidopsis thaliana.