Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box

Proc Natl Acad Sci U S A. 1988 Oct;85(19):7322-6. doi: 10.1073/pnas.85.19.7322.

Abstract

The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. By probing a phage lambda gt11 library with double-stranded oligonucleotides, we have directly isolated a cDNA encoding a Y box-binding protein designated YB-1. YB-1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a Mr of 35,414, contains 18% basic residues, and contains putative nuclear localization signals. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins*
  • DNA / analysis*
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / genetics*
  • HLA-DR Antigens / genetics*
  • Molecular Sequence Data
  • Molecular Weight
  • NFI Transcription Factors
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • RNA, Messenger / analysis
  • Transcription Factors*
  • Y-Box-Binding Protein 1

Substances

  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • HLA-DR Antigens
  • NFI Transcription Factors
  • Nuclear Proteins
  • RNA, Messenger
  • Transcription Factors
  • Y-Box-Binding Protein 1
  • YBX1 protein, human
  • DNA

Associated data

  • GENBANK/J03827