A NIR Turn-on Fluorescent Sensor For Detection of Chloride Ions in vitro and in vivo

Fengyuan Zhang; Chen Ma; Zhijuan Jiao; Shuai Mu; Yida Zhang; Xiaoyan Liu; Haixia Zhang

doi:10.1016/j.saa.2019.117729

A NIR Turn-on Fluorescent Sensor For Detection of Chloride Ions in vitro and in vivo

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 5:228:117729. doi: 10.1016/j.saa.2019.117729. Epub 2019 Oct 31.

Authors

Fengyuan Zhang¹, Chen Ma², Zhijuan Jiao², Shuai Mu², Yida Zhang², Xiaoyan Liu², Haixia Zhang³

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China; School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China.
² State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.
³ State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China. Electronic address: zhanghx@lzu.edu.cn.

PMID: 31740122
DOI: 10.1016/j.saa.2019.117729

Abstract

As the most abundant and significant anions in biosystem, chloride ions (Cl^-) participate in many important physiological processes. Thus, designing and synthesizing of a simple, sensitive, selective and long wavelength turn-on sensor for the detection and imaging of Cl^- in vitro and in vivo is very necessary. Herein, we have developed a simple porphyrin turn-on sensor 5, 10, 15, 20-Tetrakis (4-hydroxyphenyl) porphyrin (THPP) with near infrared emission wavelength (657 nm) for sensing chloride ions with remarkable sensitivity and selectivity. The detection of chloride ions was according to metal displacement assay (MDA) under physiological condition with a detection limit of 7.5 μM, and was applied to image Cl^- in vitro and in vivo successfully.

Keywords: Chloride ions; In vitro; In vivo; NIR turn-on sensor.

MeSH terms

Animals
Chlorides / analysis*
Chlorides / blood
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Infrared Rays
Mice
Optical Imaging
Porphyrins / chemistry*
Serum / chemistry
Spectrometry, Fluorescence

Substances

Chlorides
Fluorescent Dyes
Porphyrins
5,10,15,20-tetra(4-hydroxyphenyl)porphyrin