Detection and quantification of cytosolic DNA

Methods Enzymol. 2019:629:17-33. doi: 10.1016/bs.mie.2019.07.042. Epub 2019 Aug 9.

Abstract

Cytosolic DNA sensing is emerging to be a critical component of the antitumor immune response by jumpstarting innate immune responses subsequent to the stimulation of the cGAS and STING pathway. Investigating the accumulation of DNA species in the cytosol is therefore an essential readout for promising anticancer strategies. In this chapter, we present different techniques that can be utilized to detect and quantify cytosolic DNA accumulation.

Keywords: Cytosolic DNA; DNA sensing; Innate immunity; Interferon type I; STING; cGAS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods
  • Cell Line, Tumor
  • Cytosol / chemistry*
  • Cytosol / immunology
  • DNA / isolation & purification*
  • Fluorescent Antibody Technique, Direct / instrumentation
  • Fluorescent Antibody Technique, Direct / methods
  • Humans
  • Immunity, Innate / genetics*
  • Membrane Proteins / immunology
  • Membrane Proteins / metabolism
  • Neoplasms / genetics
  • Neoplasms / immunology
  • Neoplasms / pathology*
  • Nucleotidyltransferases / immunology
  • Nucleotidyltransferases / metabolism
  • Signal Transduction / genetics
  • Signal Transduction / immunology
  • Ultracentrifugation / instrumentation
  • Ultracentrifugation / methods

Substances

  • Membrane Proteins
  • STING1 protein, human
  • DNA
  • Nucleotidyltransferases
  • cGAS protein, human