To quickly and efficiently understand the intracellular metabolic characteristics of industrial microorganisms, and to find potential metabolic engineering targets, genome-scale metabolic network models (GSMMs) as a systems biology tool, are attracting more and more attention. We review here the 20-year history of metabolic network model, analyze the research status and development of GSMMs, summarize the methods for model construction and analysis, and emphasize the applications of metabolic network model for analyzing intracellular metabolic activity of microorganisms from cellular phenotypes, and metabolic engineering. Furthermore, we indicate future development trend of metabolic network model.
为了快速、高效地理解工业微生物胞内代谢特征，寻找潜在的代谢工程改造靶点，基因组规模代谢网络模型 (GSMM) 作为一种系统生物学工具越来越受到人们的关注。文中在回顾GSMM 20 年发展历程的基础上，分析了当前GSMM 的研究现状，总结了GSMM 的构建及分析方法，从预测细胞表型和指导代谢工程两个方面阐述了GSMM 在解析工业微生物胞内代谢中的应用，并展望了GSMM 未来的发展趋势。.
Keywords: genome-scale metabolic model; intracellular metabolic activity of microorganisms; production performance; systems biology.