A bla VIM-1 positive Aeromonas hydrophila strain in a near-drowning patient: evidence for interspecies plasmid transfer within the patient

Future Microbiol. 2019 Sep:14:1191-1197. doi: 10.2217/fmb-2019-0091.

Abstract

Aim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long-read sequencing showed that all isolates carried a blaVIM-1 gene located on a 165 kb incA/C plasmid. ResFinder confirmed that the resistome of the plasmid, comprising 13 resistance genes, was identical within all isolates. Discussion: Long-read sequencing using the MinION successfully reconstructed a plasmid that was identical in all isolates, providing evidence for horizontal gene transfer of this blaVIM-1 gene carrying plasmid within the patient.

Keywords: aeromonas; carbapenemase; long-read sequencing; minion; plasmid transfer.

Publication types

  • Case Reports

MeSH terms

  • Aeromonas hydrophila / drug effects*
  • Aeromonas hydrophila / genetics*
  • Carbapenems / pharmacology*
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial / genetics
  • Gene Transfer, Horizontal*
  • Genome, Bacterial
  • Gram-Negative Bacterial Infections / diagnosis
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Near Drowning
  • Plasmids / genetics*
  • Pneumonia / diagnosis
  • Pneumonia / microbiology
  • Sequence Analysis, DNA
  • Whole Genome Sequencing
  • beta-Lactamases / genetics*

Substances

  • Carbapenems
  • DNA, Bacterial
  • beta-Lactamases