Complete Genome Sequence of Clostridioides difficile Ribotype 255 Strain Mta-79, Assembled Using Oxford Nanopore and Illumina Sequencing

Jennifer K Spinler; Anne J Gonzales-Luna; Sabeen Raza; Jessica K Runge; Ruth Ann Luna; Tor C Savidge; Kevin W Garey

doi:10.1128/MRA.00935-19

Complete Genome Sequence of Clostridioides difficile Ribotype 255 Strain Mta-79, Assembled Using Oxford Nanopore and Illumina Sequencing

Microbiol Resour Announc. 2019 Oct 17;8(42):e00935-19. doi: 10.1128/MRA.00935-19.

Authors

Jennifer K Spinler^{1

2}, Anne J Gonzales-Luna³, Sabeen Raza^{4

2}, Jessica K Runge^{4

2}, Ruth Ann Luna^{4

2}, Tor C Savidge^{4

2}, Kevin W Garey³

Affiliations

¹ Department of Pathology & Immunology, Baylor College of Medicine, Houston, Texas, USA spinler@bcm.edu.
² Texas Children's Microbiome Center, Department of Pathology, Texas Children's Hospital, Houston, Texas, USA.
³ University of Houston College of Pharmacy, Houston, Texas, USA.
⁴ Department of Pathology & Immunology, Baylor College of Medicine, Houston, Texas, USA.

Abstract

Hybrid de novo assembly of Illumina/Nanopore sequence data produced a complete circular sequence of the chromosome for a Clostridioides difficile ribotype 255 (RT255) isolate from an elderly patient with recurrent C. difficile infection (CDI). This provides a high-quality representative sequence for the RT255 lineage.

Grants and funding

U01 AI124290/AI/NIAID NIH HHS/United States