Clinicopathological relevance of NFκB1/p50 nuclear immunoreactivity and its relationship with the inflammatory environment of uveal melanoma

Exp Mol Pathol. 2019 Dec:111:104313. doi: 10.1016/j.yexmp.2019.104313. Epub 2019 Sep 15.

Abstract

Purpose: To analyze the activation of NFκB1/p50 in the inflammatory and non-inflammatory environment of uveal melanoma and its association with clinicopathological factors and patient outcome.

Methods: Activation of NFκB1/p50 was evaluated in 75 cases of uveal melanoma by immunohistochemistry. mRNA expression in 58 fresh UM specimen was measured by quantitative reverse-transcriptase PCR (qRT-PCR). Western blotting was performed to validate the immunohistochemistry results in representative cases.

Results: Forty-five cases showed both cytoplasmic and nuclear immunoreactivity of NFκB1/p50. Increased level of NFκB1/p50 activation was more frequent in the inflammatory environment group as compared to non-inflammatory environment group at both transcriptional and translational level. In multivariate analysis, infiltrating macrophages and nuclear immunoreactivity of NFκB1/p50 (p < .05) in tumor cells were found to be an independent prognostic factor for poor survival.

Conclusion: Our results suggest that nuclear immunoreactivity NFκB1/p50 may serve as a useful marker in assessing the prognosis of uveal melanoma patients.

Keywords: Inflammatory environment; NFκB1/p50; Uveal melanoma; mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism*
  • Female
  • Humans
  • Inflammation / immunology
  • Inflammation / metabolism
  • Inflammation / pathology*
  • Male
  • Melanoma / immunology
  • Melanoma / metabolism
  • Melanoma / pathology*
  • Middle Aged
  • NF-kappa B p50 Subunit / genetics
  • NF-kappa B p50 Subunit / metabolism*
  • Prognosis
  • Prospective Studies
  • Survival Rate
  • Uveal Melanoma
  • Uveal Neoplasms / immunology
  • Uveal Neoplasms / metabolism
  • Uveal Neoplasms / pathology*
  • Young Adult

Substances

  • NF-kappa B p50 Subunit
  • NFKB1 protein, human