Impact of specimen storage temperature and time on the implementation of GeneXpert® testing for sexually transmitted infections in resource-constraint settings

Liteboho D Maduna; Marleen M Kock; Andrew Medina-Marino; Jeffrey D Klausner; Remco P H Peters

doi:10.1016/j.mimet.2019.105719

Impact of specimen storage temperature and time on the implementation of GeneXpert® testing for sexually transmitted infections in resource-constraint settings

J Microbiol Methods. 2019 Oct:165:105719. doi: 10.1016/j.mimet.2019.105719. Epub 2019 Sep 10.

Authors

Liteboho D Maduna¹, Marleen M Kock², Andrew Medina-Marino³, Jeffrey D Klausner⁴, Remco P H Peters⁵

Affiliations

¹ Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.
² Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa; Tshwane Academic Division, National Health Laboratory Service, Pretoria, South Africa.
³ Research Unit, Foundation for Professional Development, Pretoria, South Africa.
⁴ David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, USA; Department of Epidemiology, National Health Laboratory Services, University of California-Los Angeles, Los Angeles, CA, USA.
⁵ Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa; Anova Health Institute, Johannesburg, South Africa; Department of Medical Microbiology, School of Public Health & Primary Care (CAPHRI), Maastricht University Medical Centre, Maastricht, The Netherlands. Electronic address: rph.peters@gmail.com.

Abstract

Implementation of routine laboratory diagnostics is imperative to address the high burden of sexually transmitted infections (STI) in Sub-Saharan Africa. We demonstrate that logistical challenges of specimen storage, temperature and transport time are unlikely to impact on performance of routine STI diagnostics using the GeneXpert® platform implemented in these settings.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Africa South of the Sahara
Chlamydia trachomatis / isolation & purification*
Female
Humans
Neisseria gonorrhoeae / isolation & purification*
Sexually Transmitted Diseases, Bacterial / diagnosis*
Specimen Handling / methods*
Temperature
Trichomonas vaginalis / isolation & purification*
Urine / microbiology*

Grants and funding

R21 HD084274/HD/NICHD NIH HHS/United States