An enzymatic assay for quantification of inositol in human term placental tissue

Anal Biochem. 2019 Dec 1:586:113409. doi: 10.1016/j.ab.2019.113409. Epub 2019 Aug 27.

Abstract

A modified sensitive, cheap and simple enzymatic assay method is described for the quantitation of inositol (6-carbon polyol) in human placental tissue. Water-soluble and total (water-soluble and lipid-bound) inositol isomers were extracted and quantified using a 96-well adaptation of the Megazyme® assay. This assay specifically recognized myo-inositol (predominant isomer), d-chiro-, epi-, and allo-inositols, but not scyllo-inositol, glucose or fucose. In term placenta, water-soluble and total inositol contents were high [489 (±58) and 635 (±69) μg/g respectively], and reliably quantified with good reproducibility. This modified assay could facilitate placental inositol biology research, particularly pertinent now with interest in myo-inositol supplementation for gestational diabetes (GDM) prevention.

Keywords: Enzymatic assay; Human; Inositol; Myo-inositol; Placenta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enzyme Assays*
  • Female
  • Humans
  • Inositol / analysis*
  • Inositol / metabolism
  • Placenta / chemistry*
  • Placenta / metabolism
  • Pregnancy
  • Sugar Alcohol Dehydrogenases / metabolism*

Substances

  • Inositol
  • Sugar Alcohol Dehydrogenases
  • myo-inositol 2-dehydrogenase