Phosphorylation dependent α-synuclein degradation monitored by in-cell NMR

Wenwen Zheng; Zeting Zhang; Yansheng Ye; Qiong Wu; Maili Liu; Conggang Li

doi:10.1039/c9cc05662a

Phosphorylation dependent α-synuclein degradation monitored by in-cell NMR

Chem Commun (Camb). 2019 Sep 17;55(75):11215-11218. doi: 10.1039/c9cc05662a.

Authors

Wenwen Zheng¹, Zeting Zhang, Yansheng Ye, Qiong Wu, Maili Liu, Conggang Li

Affiliation

¹ Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China. conggangli@wipm.ac.cn.

PMID: 31469130
DOI: 10.1039/c9cc05662a

Abstract

Here we report the dephosphorylation and proteolysis of phosphorylated α-synuclein, a Parkinson's disease-related protein, in living cells in a time resolved manner using in-cell NMR.

MeSH terms

Animals
Nuclear Magnetic Resonance, Biomolecular*
Oocytes / chemistry
Oocytes / metabolism*
Phosphorylation
Proteolysis
Xenopus laevis
alpha-Synuclein / chemistry
alpha-Synuclein / metabolism*

Substances

alpha-Synuclein