Acid treatment of plasma for the inactivation of plasminogen activator inhibitor-1 (PAI-1)

M P de Maat; C Kluft; K de Boer; E A Knot; A F Jie

doi:10.1016/0049-3848(88)90026-6

Acid treatment of plasma for the inactivation of plasminogen activator inhibitor-1 (PAI-1)

Thromb Res. 1988 Dec 1;52(5):425-30. doi: 10.1016/0049-3848(88)90026-6.

Authors

M P de Maat¹, C Kluft, K de Boer, E A Knot, A F Jie

Affiliation

¹ Dept. of Internal Medicine II, University Hospital Dijkzigt, Rotterdam, The Netherlands.

PMID: 3146821
DOI: 10.1016/0049-3848(88)90026-6

Abstract

The present study was initiated to assess the effectiveness of various acid treatments of blood or plasma in the inactivation of PAI-1. It was shown that a frequently used treatment of blood or plasma with 1 M acetate buffer, pH 3.9, only partially inactivated PAI-1. The inactivation of PAI-1 in plasma was found to depend upon pH and temperature, showing an optimal inactivation at a pH less than or equal to 3, at 37 degrees C.

MeSH terms

Acetates
Acetic Acid
Glycoproteins / antagonists & inhibitors*
Glycoproteins / blood*
Humans
Hydrochloric Acid
Hydrogen-Ion Concentration
In Vitro Techniques
Plasminogen Activators / antagonists & inhibitors*
Plasminogen Inactivators*
Temperature
Tissue Plasminogen Activator / blood

Substances

Acetates
Glycoproteins
Plasminogen Inactivators
Plasminogen Activators
Tissue Plasminogen Activator
Acetic Acid
Hydrochloric Acid